1 Sathyendra V, Darowish M. Basic science of bone healing. Hand clinics. 2013;29(4):473–81.
2 Oryan A, Monazzah S, Bigham-Sadegh A. Bone injury and fracture healing biology. Biomedical and environmental sciences. 2015;28(1):57–71.
3 Florencio-Silva R, Sasso GRdS, Sasso-Cerri E, Simões MJ, Cerri PS. Biology of bone tissue: structure, function, and factors that influence bone cells. BioMed research international. 2015;2015(1):421746.
4 Loi F, Córdova LA, Pajarinen J, Lin T-h, Yao Z, Goodman SB. Inflammation, fracture and bone repair. Bone. 2016;86:119–30.
5 Schini M, Vilaca T, Gossiel F, Salam S, Eastell R. Bone turnover markers: basic biology to clinical applications. Endocrine Reviews. 2023;44(3):417–73.
6 Coucke B, Dilissen E, Cremer J, Schrijvers R, Theys T, Van Gerven L. Leukocyte-and Platelet-Rich Fibrin for enhanced tissue repair: an in vitro study characterizing cellular composition, growth factor kinetics and transcriptomic insights. Molecular Biology Reports. 2024;51(1):954.
7 Călin C, Dima O, Ancuta DL, Coman C, Pameijer CH, Paulsen F, et al. Guided bone regeneration using beta-tricalcium phosphate and leucocyte platelet-rich fibrin versus a novel biodegradable urethane composite in critical-size osseous defects in rabbit tibia: histologic results of a pilot study. Annals of Anatomy-Anatomischer Anzeiger. 2025:152672.
8 Nauth A, Schemitsch E, Norris B, Nollin Z, Watson JT. Critical-size bone defects: is there a consensus for diagnosis and treatment? Journal of orthopaedic trauma. 2018;32:S7–S11.
9 Mudalal M, Wang Z, Mustafa S, Liu Y, Wang Y, Yu J, et al. Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on tissue regeneration and proliferation of human gingival fibroblast cells cultured using a modified method. Tissue Engineering and Regenerative Medicine. 2021;18(5):895–904.
10 Xu J, Ren H, Zhao S, Li Q, Li C, Bao G, et al. Comparative effectiveness of hyaluronic acid, platelet-rich plasma, and platelet-rich fibrin in treating temporomandibular disorders: a systematic review and network meta-analysis. Head & Face Medicine. 2023;19(1):39.
11 Miron RJ, Fujioka‐Kobayashi M, Sculean A, Zhang Y. Optimization of platelet‐rich fibrin. Periodontology 2000. 2024;94(1):79–91.
12 Egierska D, Perszke M, Mazur M, Duś-Ilnicka I. Platelet-rich plasma and platelet-rich fibrin in oral surgery: A narrative review. Dental and Medical Problems. 2023;60(1):177–86.
13 Moraes RP, Costa FWG, SILVA PGdB, Carvalho FSR, Paz JERM, Matos GC, et al. Impact of L-PRF on pain and healing outcomes in lower third molar surgery: a randomized split-mouth trial. Brazilian Oral Research. 2024;38:e089.
14 Demirok SO, Eroglu CN, Koc A. Comprehensive analysis of bone tissue in extraction sockets of third molars after leukocyte and platelet rich fibrin and photobiomodulation applications. Clinical Oral Investigations. 2024;28(9):483.
15 Chmielewski M, Pilloni A, Adamska P. Application of Advanced Platelet-Rich Fibrin in Oral and Maxillo-Facial Surgery: A Systematic Review. Journal of Functional Biomaterials. 2024;15(12):377.
16 Almutairi M, Alshammari SZ, Almutairi D, Alansari TA, Aldhafiri F, Almutairi AN, et al. Leukocyte-Platelet-Rich Fibrin Combined With Demineralized Bovine Bone Mineral for Maxillary Sinus Augmentation: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Cureus. 2025;17(6).
17 Medeiros-Monzón A, Blanco-Carrión A, Gándara-Vila P, Camolesi G, Pérez-Jardón A, Lorenzo-Pouso AI, et al. Efficacy of leukocyte-and platelet-rich fibrin in the treatment and prevention of medication-related osteonecrosis of the jaw: a prospective study. Medicina oral, patologia oral y cirugia bucal.27249.
18 Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2006;101(3):e37–e44.
19 A Zumstein M, Berger S, Schober M, Boileau P, W Nyffeler R, Horn M, et al. Leukocyte-and platelet-rich fibrin (L-PRF) for long-term delivery of growth factor in rotator cuff repair: review, preliminary results and future directions. Current pharmaceutical biotechnology. 2012;13(7):1196–206.
20 Van Cleemput T, Hendrikx S, Politis C, Spaey Y. Leukocyte-and platelet-rich fibrin: a new method for scalp defect reconstruction. Dermatologic Surgery. 2022;48(2):261–2.
21 Sadeghian A, Rohani B, Hasannia S, Mashhadiabbas F, Fekrazad R. Synergistic Effect of Photobiomodulation and Leukocyte-Platelet Rich Fibrin on Bone Regeneration in Rat Critical Defects. Photodiagnosis and Photodynamic Therapy. 2025:104687.
22 Khanbazi M, Bigham-Sadegh A, Oryan A, Meimandi-Parizi A, Jannesar A. The effects of allogeneic and xenogeneic lyophilized leukocyte-and platelet-rich fibrin on bone healing in rat. Injury. 2024;55(3):111396.
23 Crisci A, Lombardi D, Serra E, Lombardi G, Cardillo F, Crisci M. Standardized protocol proposed for clinical use of L-PRF and the use of L-PRF Wound Box®. Journal of Unexplored Medical Data¦ Volume. 2017;2:78.
24 Wang Z, Han L, Sun T, Wang W, Li X, Wu B. Preparation and effect of lyophilized platelet-rich fibrin on the osteogenic potential of bone marrow mesenchymal stem cells in vitro and in vivo. Heliyon. 2019;5(10).
25 Lane JM, Sandhu HS. Current approaches to experimental bone grafting. Orthopedic Clinics of North America. 1987;18(2):213–25.
26 Emery SE, Brazinski MS, Koka A, Bensusan JS, Stevenson S. The biological and biomechanical effects of irradiation on anterior spinal bone grafts in a canine model. JBJS. 1994;76(4):540–8.
27 Ehrenfest DMD, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte-and platelet-rich fibrin (L-PRF). Trends in biotechnology. 2009;27(3):158–67.
28 Tablin F, Walker NJ, Hogle SE, Pratt SM, Norris JW. Assessment of platelet growth factors in supernatants from rehydrated freeze-dried equine platelets and their effects on fibroblasts in vitro. American journal of veterinary research. 2008;69(11):1512–9.
29 Dohan Ehrenfest DM, Pinto NR, Pereda A, Jiménez P, Corso MD, Kang B-S, et al. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte-and platelet-rich fibrin (L-PRF) clot and membrane. Platelets. 2018;29(2):171–84.
30 INCHINGOLO F, Tatullo M, Marrelli M, Inchingolo A, SCACCO S, Inchingolo A, et al. Trial with Platelet-Rich Fibrin and Bio-Oss used as grafting materials in the treatment of the severe maxillar bone atrophy: clinical and radiological evaluations. European Review for Medical & Pharmacological Sciences. 2010;14(12).
31 Quirynen M, Blanco J, Wang HL, Donos N, Temmerman A, Castro A, et al. Instructions for the use of L‐PRF in different clinical indications. Periodontology 2000. 2025;97(1):420–32.
32 Ehrenfest DMD, Diss A, Odin G, Doglioli P, Hippolyte M-P, Charrier J-B. In vitro effects of Choukroun's PRF (platelet-rich fibrin) on human gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts in primary cultures. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2009;108(3):341–52.
33 Ehrenfest DMD, Doglioli P, de Peppo GM, Del Corso M, Charrier J-B. Choukroun's platelet-rich fibrin (PRF) stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way. Archives of oral biology. 2010;55(3):185–94.
34 Chang IC, Tsai CH, Chang YC. Platelet‐rich fibrin modulates the expression of extracellular signal‐regulated protein kinase and osteoprotegerin in human osteoblasts. Journal of biomedical materials research Part A. 2010;95(1):327–32.
35 Clipet F, Tricot S, Alno N, Massot M, Solhi H, Cathelineau G, et al. In vitro effects of Choukroun's platelet-rich fibrin conditioned medium on 3 different cell lines implicated in dental implantology. Implant dentistry. 2012;21(1):51–6.
36 Everts PA, Lana JF, Alexander RW, Dallo I, Kon E, Ambach MA, et al. Profound properties of protein-rich, platelet-rich plasma matrices as novel, multi-purpose biological platforms in tissue repair, regeneration, and wound healing. International Journal of Molecular Sciences. 2024;25(14):7914.
37 Pinto N, Yu J, Koirala S, Mourão CF, Andrade C, Rescigno E, et al. L‐PRF in extra‐oral wound care. Periodontology 2000. 2025;97(1):342–62.
38 Li Q, Reed DA, Min L, Gopinathan G, Li S, Dangaria SJ, et al. Lyophilized platelet-rich fibrin (PRF) promotes craniofacial bone regeneration through Runx2. International journal of molecular sciences. 2014;15(5):8509–25.
39 Miron RJ, Moraschini V, Estrin NE, Shibli JA, Cosgarea R, Jepsen K, et al. Periodontal Regeneration using Platelet‐Rich Fibrin. Furcation defects: a systematic review with meta‐analysis. Periodontology 2000. 2025;97(1):191–214.
40 Coucke B, De Vleeschouwer S, van Loon J, Van Calenbergh F, Van Hoylandt A, Van Gerven L, et al. Leukocyte-and platelet-rich fibrin in cranial surgery: a single-blinded, prospective, randomized controlled noninferiority trial. Journal of Neurosurgery. 2024;141(2):500–8.
41 Strauss F-J, Nasirzade J, Kargarpoor Z, Stähli A, Gruber R. Effect of platelet-rich fibrin on cell proliferation, migration, differentiation, inflammation, and osteoclastogenesis: a systematic review of in vitro studies. Clinical oral investigations. 2020;24(2):569–84.
42 Omar YK, Rashidy MAE, Ahmed GB, Aboulela AG. Evaluation of leukocyte-platelet rich fibrin as an antibiotic slow-release biological device in the treatment of moderate periodontitis: a randomized controlled clinical trial. BMC Oral Health. 2024;24(1):1530.
43 Blanco J, García A, Hermida‐Nogueira L, Castro AB. How to explain the beneficial effects of leukocyte‐and platelet‐rich fibrin. Periodontology 2000. 2025;97(1):74–94.
44 Faot F, Deprez S, Vandamme K, Camargos GV, Pinto N, Wouters J, et al. The effect of L-PRF membranes on bone healing in rabbit tibiae bone defects: micro-CT and biomarker results. Scientific reports. 2017;7(1):46452.
45 Idulhaq M, Mudigdo A, Utomo P, Wasita B, Warman FI. Platelet-rich fibrin as a tissue engineering material in accelerate bone healing in rat bone defects: A systematic review and meta-analysis. Annals of Medicine and Surgery. 2022;84:104869.
46 Júnior JAG, Nóbrega F, Oliveira PG, Bergamo ET, Cadore U, Gomes MZdV, et al. Evaluation of Implant Surface Modification with Nanohydroxyapatite Associated with the Use of L-PRF: In Vivo Study in Rats. Journal of Functional Biomaterials. 2023;14(7):370.
47 Jensen SS, Broggini N, Hjørting‐Hansen E, Schenk R, Buser D. Bone healing and graft resorption of autograft, anorganic bovine bone and β‐tricalcium phosphate. A histologic and histomorphometric study in the mandibles of minipigs. Clinical oral implants research. 2006;17(3):237–43.
48 Marino JT, Ziran BH. Use of solid and cancellous autologous bone graft for fractures and nonunions. Orthopedic Clinics. 2010;41(1):15–26.
49 Azi ML, Aprato A, Santi I, Kfuri Jr M, Masse A, Joeris A. Autologous bone graft in the treatment of post-traumatic bone defects: a systematic review and meta-analysis. BMC Musculoskeletal Disorders. 2016;17(1):465.
50 Farshidfar N, Amiri MA, Estrin NE, Ahmad P, Sculean A, Zhang Y, et al. Platelet‐rich plasma (PRP) versus injectable platelet‐rich fibrin (i‐PRF): A systematic review across all fields of medicine. Periodontology 2000. 2025.
51 Ravi S, Santhanakrishnan M. Mechanical, chemical, structural analysis and comparative release of PDGF-AA from L-PRF, A-PRF and T-PRF-an in vitro study. Biomaterials Research. 2020;24(1):16.
