Document Type : Original Article
Abstract
Background: Elevated free radical generation in inflamed joints and impaired antioxidant system has been implicated in rheumatoid arthritis (RA). Evidence suggests that exercise improves the symptoms of rheumatoid arthritis, although the mechanism is not well understood. The aim of this study was to investigate the effect of swimming training on the glutathione antioxidant system and oxidative changes induced by rheumatoid arthritis in rats. Material and Methods: 8-week-old female Wistar rats were distributed within three groups (n=10 in each): a normal control group (C), a rheumatoid arthritis control group (RA+C), and a rheumatoid arthritis swimming training group (RA+EX). At nine weeks-of-age, rheumatoid arthritis was induced by injection of complete Freundchr('39')s adjuvant (20mg/kg) into the tail of Wistar rats. The degree of arthritis was assessed by macroscopic assessments such as walking ability, skin redness, and swelling in the joints. The RA+EX rats were conditioned to swim for the 4-week period (~20-60 min/day), whereas the C and RA+C remained sedentary in their cages. 48 hours after the last training session, a venous sample was collected to determine metabolic parameters, including glutathione peroxidase, glutathione reductase and lipid peroxidation products (malondialdehyde).The variance analysis test and the Tukey post-hoc test were applied to analyze the data (p < 0.05). Results: As predicted, rheumatoid arthritis significantly increased levels of malondialdehyde, and swimming training prevented this response (p < 0.05). Interestingly, glutathione peroxidase was significantly decreased in the RA+C group compared to the RA+EX and C groups (p < 0.05). In addition, the severity of rheumatoid arthritis clinical signs in RA+EX group was significantly lower than RA+C group. (p < 0.05). Conclusion: Our findings suggest that swimming training may be useful in preventing the negative changes in glutathione antioxidant system and oxidative stress parameters related to rheumatoid arthritis.
Keywords
- Wang L, Gao C, Zhu D, Chen LH. Effect of functional exercises on patients with rheumatoid arthritis: a meta-analysis. Beijing Da Xue Xue Bao Yi Xue Ban. 2018; 50(6):991-7.
- Narváez J. Treatment of rheumatoid arthritis. Med Clin (Barc). 2016; 147(4):176-80.
- da Fonseca LJS, Nunes-Souza V, Goulart MOF, Rabelo LA. Oxidative stress in rheumatoid arthritis: what the future might hold regarding novel biomarkers and add-on therapies. Oxid Med Cell Longev. 2019; 1:7536805-11.
- Mateen S, Moin S, Khan AQ, Zafar A, Fatima N. Increased reactive oxygen species formation and oxidative stress in rheumatoid arthritis. PLoS One. 2016; 11(4):0152925-32.
- Attia AM, Ibrahim FA, Abd El-Latif NA, Aziz SW. Therapeutic antioxidant and anti-inflammatory effects of laser acupuncture on patients with rheumatoid arthritis. Lasers Surg Med. 2016; 48(5):490-7.
- Kadry MO. Liposomal glutathione as a promising candidate for immunological rheumatoid arthritis therapy. Heliyon. 2019; 5(7):e02162-9.
- Metsios GS, Kitas GD. Physical activity, exercise and rheumatoid arthritis: Effectiveness, mechanisms and implementation. Best Pract Res Clin Rheumatol. 2018; 32(5):669-82.
- Lange E, Kucharski D, Svedlund S, Svensson K, Bertholds G. Effects of aerobic and resistance exercise in older adults with rheumatoid arthritis: a randomized controlled trial. Arthritis Care Res (Hoboken). 2019; 71(1):61-70.
- Powers SK, Deminice R, Ozdemir M, Yoshihara T, Bomkamp MP, Hyatt H. Exercise-induced oxidative stress: friend or foe? J Sport Health Sci. 2020; 9(5):415-25.
- Rall LC, Roubenoff R, Meydani SN, Han SN, Meydani M. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a marker of oxidative stress in rheumatoid arthritis and aging: effect of progressive resistance training. J Nutr Biochem. 2000; 11(11-12):581-4.
- Wadley AJ, Veldhuijzen van Zanten JJ, Stavropoulos-Kalinoglou A. Three months of moderate-intensity exercise reduced plasma 3-nitrotyrosine in rheumatoid arthritis patients. Eur J Appl Physiol. 2014; 114(7):1483-92.
- Asquith DL, Miller AM, McInnes IB, Liew FY. Animal models of rheumatoid arthritis. Eur J Immunol. 2009; 39(8):2040-4.
- Kalantari A, Saremi A, Shavandi N, Foroutan Nia A. Impact of four week swimming exercise with alpha-tocopherol supplementation on fertility potential in healthy rats. Urol J. 2017; 14(5):5023-6.
- Fischer BD, Adeyemo A, O'Leary ME, Bottaro A. Animal models of rheumatoid pain: experimental systems and insights. Arthritis Res Ther. 2017; 19(1):146-52.
- González-Chávez SA, Quiñonez-Flores CM, Espino-Solís GP. Exercise exacerbates the transcriptional profile of hypoxia, oxidative stress and inflammation in rats with adjuvant-induced arthritis. Cells. 2019; 8(12):1493-9.
- Burmester GR, Pope JE. Novel treatment strategies in rheumatoid arthritis. Lancet. 2017; 389(10086):2338-48.
- Phull AR, Nasir B, Haq IU, Kim SJ. Oxidative stress, consequences and ROS mediated cellular signaling in rheumatoid arthritis. Chem Biol Interact. 2018; 281:121-36.
- Moosavian SP, Paknahad Z, Habibagahi Z. A randomized, double-blind, placebo-controlled clinical trial, evaluating the garlic supplement effects on some serum biomarkers of oxidative stress, and quality of life in women with rheumatoid arthritis. Int J Clin Pract. 2020; 74(7):13498-503.
- Shan L, Tong L, Hang L, Fan H. Fangchinoline supplementation attenuates inflammatory markers in experimental rheumatoid arthritis-induced rats. Biomed Pharmacother. 2019;111:142-50.
- Hassan MQ, Hadi RA, Al-Rawi ZS, Padron VA, Stohs SJ. The glutathione defense system in the pathogenesis of rheumatoid arthritis. J Appl Toxicol. 2001; 21(1):69-73.
- Sindhu G, Ratheesh M, Shyni GL, Nambisan B, Helen A. Anti-inflammatory and antioxidative effects of mucilage of Trigonella foenum graecum (Fenugreek) on adjuvant induced arthritic rats. Int Immunopharmacol. 2012; 12(1):205-11.
- Powers SK, Radak Z, Ji LL. Exercise-induced oxidative stress: past, present and future. J Physiol. 2016; 594(18):5081-92.
- Guaraldo SA, Serra AJ, Amadio EM, Antônio EL, Silva F. Lasers Med Sci. 2016; 31(5):833-40. Lasers Med Sci. 2016; 31(5):833-40.
- Nonato LF, Rocha-Vieira E, Tossige-Gomes R, Soares AA, Soares BA, Freitas DA, Oliveira MX, Mendonca VA, Lacerda AC, Massensini AR, Leite HR. Swimming training attenuates oxidative damage and increases enzymatic but not non-enzymatic antioxidant defenses in the rat brain. Braz J Med Biol Res. 2016; 49:5310-16.
- Tuna Z, Duger T, Atalay-Guzel N, Aral A, Basturk B, Haznedaroglu S, Goker B. Aerobic exercise improves oxidant-antioxidant balance in patients with rheumatoid arthritis. J Phys Ther Sci. 2015; 27:1239–42.
- Dos Santos SA, Dos Santos Vieira MA, Simões MCB. Photobiomodulation therapy associated with treadmill training in the oxidative stress in a collagen-induced arthritis model. Lasers Med Sci. 2017; 32(5):1071-79.
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