Authors
- nader tanideh
- Mohammad Amin Rostami Nasab
- Ida Hasssanpour
- Omid Koohi Hosseinabadi
- Maryam Mojahed
- Sara Sadat Nabavizadeh
Abstract
Background: Osteoarthritis (OA) is widespread degenerative joint disease. Although many therapeutic policies exist, no clear preventive remedy exists. Due to various side effects caused by conventional medication; we aim to evaluate the effect of leaf of Psidium guajava essential oil on animal model of OA.
Methods: This 8-week animal model study was conducted on 25 male rats with induced osteoarthritis with collagenase. The rats were divided into 5 groups randomly, the first group that serves as negative control group and received intra-articular injections of saline, the second group received olive oil, the third group intra articular(IA)Hyalgan, the fourth group received low dosage (25 λ) essential oil of Psidium guajava L. leaf, and the last group which received high dosage (50 λ) essential oil of Psidium guajava L. leaf. All groups received their medication for 4 weeks. After 8 weeks from beginning of study, all of the animals were euthanized with CO2 and samples harvested. All the samples underwent histopathological and radiological evaluation and data were collected and analyzed by Kruskal-Wallis nonparametric test (distribution free) by SPSS version 23.00.
Results: In histopathological findings illustrate a considerable difference between high dose (50λ) treatment group and the control group. Moreover; in radiographic findings, significant difference was observed between groups in evaluation of distribution in joint space width. (P value =0.0001)
Conclusion: Essential oil of Psidium guajava L. leaf has a considerable positive effect on osteoarthritis.
Trial registration: 94-01-01-9732
Keywords
on osteoarthritis: update on clinical evidence and
molecular mechanisms. Osteoarthritis Cartilage. 2015;
23(6):841-50.
2.Srikanth VK, Fryer JL, Zhai G, Winzenberg TM,
Hosmer D, Jones G. A meta-analysis of sex differences
prevalence, incidence and severity of osteoarthritis.
Osteoarthritis Cartilage. 2005;13(9):769-81.
3.Brown TD, Johnston RC, Saltzman CL, Marsh JL,
Buckwalter JA. Posttraumatic osteoarthritis: a first
estimate of incidence, prevalence, and burden of
disease. J Orthop Trauma. 2006;20(10):739-44.
4.O'Neil CK, Hanlon JT, Marcum ZA. Adverse effects of
analgesics commonly used by older adults with
osteoarthritis: focus on non-opioid and opioid
analgesics. Am J Geriatr Pharmacother. 2012;10(6):331-
42.
5.Juhl C, Christensen R, Roos EM, Zhang W, Lund H.
Impact of exercise type and dose on pain and disability
in knee osteoarthritis: a systematic review and meta-
regression analysis of randomized controlled trials.
Arthritis Rheumatol. 2014;66(3):622-36.
6.Wojdasiewicz P, Poniatowski LA, Szukiewicz D. The
role of inflammatory and anti-inflammatory cytokines in
the pathogenesis of Osteoarthritis. Mediators Inflamm.
2014;27(7):317-23.
7.Santos FA, Rao VSN, Silveria ER. Investigations on the
antinociceptive effect of Psidium guajava leaf essential
oil and its major constituents. Phytotherapy research.
1998;12(1):24-7.
8.Olajide OA, Awe SO, Makinde JM. Pharmacological
studies on the leaf of Psidium guajava. Fitoterapia.
1999;70(1):25-31
Glasson SS. Osteoarthritis severity is sex dependent in a
surgical mouse model. Osteoarthritis Cartilage.
2007;15(6):695-700.
10.Chen B, Deng Y, Tan Y, Qin J, Chen LB. Association
between severity of knee osteoarthritis and serum and
synovial fluid interleukin 17 concentrations. J Int Med
Res. 2014;42(1):138-44.
11.Alshami AM. Knee osteoarthritis related pain: a
narrative review of diagnosis and treatment. Int J Health
Sci (Qassim). 2014;8(1):85-104.
12.Sakthivel KM, Guruvayoorappan C. Amentoflavone
inhibits iNOS, COX-2 expression and modulates cytokine
profile, NF-kappaB signal transduction pathways in rats
with ulcerative colitis. Int Immunopharmacol.
2013;17(3):907-16.
13.Soman S, Rajamanickam C, Rauf AA, Indira M.
Beneficial effects of Psidium guajava leaf extract on
diabetic myocardium. Exp Toxicol Pathol. 2013;65(1-
2):91-5.
14.Cameron M, Gagnier JJ, Little CV, Parsons TJ, Blumle
A, Chrubasik S. Evidence of effectiveness of herbal
medicinal products in the treatment of arthritis. Part 2:
Rheumatoid arthritis. Phytother Res. 2009;23(12):1647-
62.
15.Willey JS, Long DL, Vanderman KS, Loeser RF.
Ionizing radiation causes active degradation and reduces
matrix synthesis in articular cartilage. Int J Radiat Biol.
2013;89(4):268-77.
16.Cameron M, Gagnier JJ, Little CV,Parsons TJ, Blumle
A, Chrubasike S. Evidence of effectiveness of herbal
medicinal products in the treatment of arthritis. Part 1:
Osteoarthritis. Phytother Res. 2009:23(11):1497-515.
دوره شانزدهم، شماره 2(شماره مسلسل 61)، بهار 1397، صفحههای 193-185 ارزیابی عصاره برگ گیاه گوارا بر استئوآرتریت القا شده ...
17.Blamey R, Jolly K, Greenfield S, Jobanputra P.
Patterns of analgesic use, pain and self-efficacy: a cross-
sectional study of patients attending a hospital
rheumatology clinic. BMC Musculoskelet Disord.
2009;10:137.
18.Zhao XZ, Li XW, Jin YR, Yu XF, Qu SC, Sui DY.
Hypolipidemic effects of kaempferide-7-O-(4''-O-
acetylrhamnosyl)-3-O-rutinoside in hyperlipidemic rats
induced by a high-fat diet. Mol Med Rep. 2012;5(3):837-
41.
19.van der Kraan PM, van den Berg WB. Osteoarthritis
in the context of ageing and evolution. Loss of
chondrocyte differentiation block during ageing. Ageing
Res Rev. 2008;7(2):106-13.
20.Martel-Pelletier J, Boileau C, Pelletier JP, Roughley
PJ. Cartilage in normal and osteoarthritis conditions.
Best Pract Res Clin Rheumatol. 2008;22(2):351-84.
21.Dutta S, Das S. A study of the anti-inflammatory
effect of the leaves of Psidium guajava Linn. on
experimental animal models. Pharmacognosy Res.
2010;2(5):313-7.
22.Huang CS, Yin MC, Chiu LC. Antihyperglycemic and
antioxidative potential of Psidium guajava fruit in
streptozotocin-induced diabetic rats. Food Chem
Toxicol. 2011;49(9):2189-95.
23.Jang M, Jeong SW, Cho SK, Ahn KS, Lee JH, Yang DC,
et al. Anti-inflammatory effects of an ethanolic extract
of guava (Psidium guajava L.) leaves in vitro and in vivo.
J Med Food. 2014;17(6):678-85.
24. Porwal K, Pal S, Dev K, China SP, Kumar Y, Singh C,
et al. Guava fruit extract and its triterpene constituents
have osteoanabolic effect: Stimulation of osteoblast
differentiation by activation of mitochondrial respiration
via the Wnt/beta-catenin signaling. J Nutr Biochem.
2017;44:22-34.
)