Mohammad Ali Hosseinian, MD; Atusa Gharib, MD; Javad Sahebi, MD; Mohsen Khandaghi, MD
Abstract
Background: Sutures and glue are the commonly used techniques in peripheral nerve repair with controversies in the results of experimental researches studying either technique. This experimental study aimed at evaluation of the axonal regeneration after end-to-side nerve repair with glue and suture ...
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Background: Sutures and glue are the commonly used techniques in peripheral nerve repair with controversies in the results of experimental researches studying either technique. This experimental study aimed at evaluation of the axonal regeneration after end-to-side nerve repair with glue and suture in rats.Methods: In an experimental study, 30 male Wistar rats were divided into three groups: group 1 (n=10) were submitted to fibular nerve transection with end-to-side nerve anastomosis using sutures alone group 2 (n=10) were submitted to fibular transection with end-to side nerve anastomosis using sutures and cyanacrylate glue in the lateral surface of an intact tibial nerve and group 3 (n=10), were submitted to surgery without repair (control group). At the end of the study (after 16 weeks) period, the three groups were evaluated by "walking track", and posterior morphometrical analysis.Results: At the end of the study, the mean weight of the groups had increased. The functional tests showed difference in the walking track analysis in groups 1 and 2, with important recovery in group 2 in walking pattern, while group 3 showed no function. Histological comparison showed no statistically significant difference between group 1 and 2, regarding the count of regenerated axons in the site of nerve repair. The number of regenerated axons, however, was more in group 2. The surgical time in group 3 was shorter than groups 1 and 2.Conclusion: End-to-side nerve repair using glue presented better results than suture following transection of fibular nerve in the rat experimental model.
Reza Erfanian Reza; Masoumeh Firouzi, PhD; Mohammad Hossein Nabian, MD; Masoud Darvishzadeh, PhD; Leila Oriadi Zanjani, MD; Shayan Abdollahzadegan; Reza Shahryar Kamrani, MD
Abstract
Background: The use of fibrin adhesive has broad background in medicine and peripheral nerve repair. The physical properties of these adhesives has restricted their usage. This experiment studied the performance and physical characteristics of fibrin glue (which is prepared from single donor human plasma) ...
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Background: The use of fibrin adhesive has broad background in medicine and peripheral nerve repair. The physical properties of these adhesives has restricted their usage. This experiment studied the performance and physical characteristics of fibrin glue (which is prepared from single donor human plasma) in the repair of posterior tibial nerve of rat.Methods: In this study, posterior tibial nerves of 40 rats were transected. In group A no repair was performed in group B repair was done by one microsuture in group C repair was done by three microsutures in group D by one suture plus fibrin adhesive and in group E repair was done just with fibrin adhesive. After 8 weeks of follow-up, Tibial Function Index and at the end of the study, adhesive strength inflammation and scar formation were measured.Results: Functional recovery in the eighth week for groups A and E were significantly lower than groups B and C (p < /em>=.03). No rat repaired with fibrin adhesive had dehiscence, while half of rats in group A had complete dehiscence- was significantly higher than the others groups (p < /em>=.002). Inflammation and scar tissue were similar between the groups.Conclusion: The produced fibrin adhesive provides required adhesive and could hold nerve stumps in long term.
