Amin Bigham-Sadegh; Saeid Lotfi; Amad Oryan; Iman Hafar
Abstract
Background: Facilitating the fracture healing process is important to increase the fracture healing speed and to decrease the time period till union. This study aimed to evaluate the effects of blood clot and doxycycline on bone healing process.Methods: Twenty mature male New Zealand white rabbits were ...
Read More
Background: Facilitating the fracture healing process is important to increase the fracture healing speed and to decrease the time period till union. This study aimed to evaluate the effects of blood clot and doxycycline on bone healing process.Methods: Twenty mature male New Zealand white rabbits were used, in this study. A defect was created in the middle part of the radius. The rabbits were randomly distributed into 4 groups and the gaps were filled with the graft materials: blood clot, doxycycline, combination of blood clot and doxycycline and control group in which the defect was left empty. Radiographs of operated limbs were taken on 14th, 28th, 42nd and 56th postoperative days. Histologic samples were taken on the 56th day post surgery.Results: On radiographic evaluation significant difference between the groups was not observed (p>0.05). On histopathological evaluation, blood clot and doxycycline groups were superior to control group (P<0.05), also combination of blood clot and doxycycline group was superior to other ones (P<0.05). There was no evidence of graft rejection in any group.Conclusion: This study demonstrated that the combination of blood clot and doxycycline has a better function in bone healing process than other groups.
Ghazaleh Moradkhani; Morad Karimpour; S Mahmoud Taheri
Abstract
Background: Porous titanium structures have recently gained considerable popularity among researchers in studies examining bone ingrowth and osseointegration. Porous implants fabricated using triply periodic minimal surface design (TPMS) and designed through 3D printing techniques exhibited remarkable ...
Read More
Background: Porous titanium structures have recently gained considerable popularity among researchers in studies examining bone ingrowth and osseointegration. Porous implants fabricated using triply periodic minimal surface design (TPMS) and designed through 3D printing techniques exhibited remarkable mechanical strength and cell viability compared to conventional implants. This study aimed to evaluate the effect of pore size of titanium implants with gyroid structure.Methods: This study was conducted on Adult male Wistar rats weighing 350 and 450 g for the animal study by the calvarial defect model to investigate bone regeneration. Three disk-shaped implants were designed using a gyroid structure with pore sizes of 400, 500, and 600 micrometers. All implants were made by additive manufacturing (Selective Laser Melting) using Ti6Al4V medical-grade powder. Animals were sacrificed after 12 weeks, the skin was removed from the calvaria, and the implants were removed for histological examination.Results: Gyroid structures had a high surface-to-volume ratio and pore connectivity, facilitating cell adhesion and ossification. A significant amount of bone ingrowth was observed in the 400 mm group, so that bone penetrated into pores significantly more than in the other groups. However, the vascularization was more pronounced in the 600 μm group than in the other groups.Conclusion: According to the results, there was a positive effect of porosity in titanium implants in encouraging bone ingrowth. The porosity size of 400 μm was more suitable for the differentiation and proliferation of bone cells and thus the osseointegration in porous titanium implants with gyroid structure.
Basic
Vahid Zarghami; Fereshteh Moharramzadeh Jeghanab; Mohammad Ali Shokrgozar
Abstract
Background: One of the issues related to stimulating and accelerating bone regeneration and bone repair is the role of the immune system in fracture healing, known as osteo-immunology.Method: In these review 57 articles of science direct and pumped database is investigated for potential role and mechanism ...
Read More
Background: One of the issues related to stimulating and accelerating bone regeneration and bone repair is the role of the immune system in fracture healing, known as osteo-immunology.Method: In these review 57 articles of science direct and pumped database is investigated for potential role and mechanism of the immune system's response to bone fracture. Results: biological materials such as tumor necrosis factor alpha (TNF-α), lipopolysaccharide (LPS), interleukin-17 (IL-17) protein, lipotic Acid (LTA) could regulate the immune system, which have the ability to improve the ossification process and faster healing.Conclusion: The direction of future research was predicted regarding the emergence of new therapeutic compounds derived from bone-building materials such as bone growth factors and substances that regulate the behaviour of the immune system in order to regenerate bone.
Ali Doostmohammadi; Mohammad Hossein Fathi; Amin Bigham Sadegh; Amir Hossein Tavakolizadeh; Hamed Akbarian
Abstract
Background: Bioactive bioceramics have been regarded by researchers in recent years. Bioglass and Hydroxyapatite (HA) are the examples of these bioactive bioceramics. Production and characterization of nano bioglass and HA for bone repair applications were the purpose of this study. Methods: Nano bioglass ...
Read More
Background: Bioactive bioceramics have been regarded by researchers in recent years. Bioglass and Hydroxyapatite (HA) are the examples of these bioactive bioceramics. Production and characterization of nano bioglass and HA for bone repair applications were the purpose of this study. Methods: Nano bioglass and HA were produced and the particles shape and size were characterized by X-ray flurescence (XRF). The bioactivity of the particles of HA and nano bioglass and combination of the two were tested, by introducing them into the cavities formed in rabbit's Tibia. The incorporation and bone induction of these 3 different paricles in bone were evaluated by radiography and also histopathology of the resected specimens. Results: The characterized tests confirmed that nano bioglass and natural HA were produced with desired composition. The radiographic and histopathologic evaluation illustrated good bioactivity of nano-size bioglass, HA and HA-bioglass composite, leading to restoration of bone defects after 4 to 6 weeks. It was also observed that bioactivity of HA-bioglass composite was superior to bioglass and HA used singularly. Conclusions: The osteoconductivty of HA and osteoinductivity of bioglass combined as a composite lead to excellent bioactivity and good response in the hard bony tissue.
