Effects of using chitosan (CHS) scaffolds on bone healing in rat animal model: radiological and histopathological evaluations

Heidari, Fatemeh; Bigham-Sadegh, Amin; Oryan, Ahmad; Meimandi-Parizi, Abdolhamid; Shaikhzadeh, Alireza; Afzali, Seyed Ali; Asgari, Maryam

doi:10.22034/ijos.2025.550195.1175

Effects of using chitosan (CHS) scaffolds on bone healing in rat animal model: radiological and histopathological evaluations

Document Type : Original Article

Authors

Fatemeh Heidari ¹

Amin Bigham-Sadegh ²

Ahmad Oryan ³

Abdolhamid Meimandi-Parizi ⁴

Alireza Shaikhzadeh ⁵

Seyed Ali Afzali ⁶

Maryam Asgari ⁶

¹ Department of Veterinary surgery and radiology

² Shiraz University

³ Professor, Department of Pathology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

⁴ . Professor, Department of surgery and Radiology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

⁵ Resident of Surgery, Faculty of Veterinary Medicine, University of Shiraz, Shiraz, Iran

⁶ Graduated in general veterinarian

https://doi.org/10.22034/ijos.2025.550195.1175

Abstract

Abstract

Introduction: Bone fracture is one of the most common bone injuries in the world. It is, therefore, not surprising that bone healing is a critical orthopedic problem. Currently, bone grafts (autografts, allografts and xenografts) have been used as bone replacement strategies. The purpose of this study was to evaluate the efficacy of application of chitosan (CHS) as an osteo-inducer in the process of bone healing.
Materials and Methods: Fifteen male rats, in the same age and weight range, were divided into three groups of 5. 3 mm of metaphysis of the radius bone of each animal was removed. The defect was left empty in the first , filled with autograft in the second ,and filled with CHS in the third group. Radiographs of the defects were obtained on 28^th and 56^th day post-operation. The animals were euthanized according to human ethics and the defects were investigated histologically on the 56^th post-operation day.
Results and Discussion: According to radiographic and histopathologic results, CHS indicated a improved effect ,and improved bone healing process in comparison with the other two treatment modalities.
Conclusion: It can be concluded that use of chitosan improves the potential for bone formation and bone healing criteria. These composite materials, however, deserve more studies.

Keywords

Chitosan

Bone healing

Radiology

Histopathology

Rat

Subjects