Iranian Journal of Orthopedic Surgery

The Effect of Simultaneous Application of Blood Clot and Doxycycline on Bone Healing in Rabbit

Document Type : Original Article

Authors

Amin Bigham-Sadegh 1
Saeid Lotfi 2
Amad Oryan 3
Iman Hafar 2

1 Orthopedic Surgeon, Orthopaedic Surgeon, Department of Veterinary Surgery and Radiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

2 Graduated DVM, Department of Veterinary Surgery and Radiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

3 Comparative Pathologist, Department of Veterinary Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

https://doi.org/10.22034/ijos.2022.330332.1019
Abstract
Background: Facilitating the fracture healing process is important to increase the fracture healing speed and to decrease the time period till union. This study aimed to evaluate the effects of blood clot and doxycycline on bone healing process.
Methods: Twenty mature male New Zealand white rabbits were used, in this study. A defect was created in the middle part of the radius. The rabbits were randomly distributed into 4 groups and the gaps were filled with the graft materials: blood clot, doxycycline, combination of blood clot and doxycycline and control group in which the defect was left empty. Radiographs of operated limbs were taken on 14th, 28th, 42nd and 56th postoperative days. Histologic samples were taken on the 56th day post surgery.
Results: On radiographic evaluation significant difference between the groups was not observed (p>0.05). On histopathological evaluation, blood clot and doxycycline groups were superior to control group (P<0.05), also combination of blood clot and doxycycline group was superior to other ones (P<0.05). There was no evidence of graft rejection in any group.
Conclusion: This study demonstrated that the combination of blood clot and doxycycline has a better function in bone healing process than other groups.
 

Keywords

Biomaterial
Blood clot
Doxycycline
Bone regeneration
Rabbit

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