Iranian Journal of Orthopedic Surgery

Evaluation of hydroalcoholic extract of Psidium Guajava Linn.(Myrtaceae) leave effect with synovium- derived Stem Cells and Platelet Rich Plasma (PRP) on induced osteoarthritis of the knee in Male Rat

Authors

S Saravani
N Tanideh
M Fazeli
N Azarpira
E Nadimi
M Lotfi
Ayda Iraji
Sh Zareh
https://doi.org/10.22034/ijos.2020.121332
Abstract
Background: Osteoarthritis (OA) recognized, as degenerative arthritis. Synthetic drugs available to treat the OA  have, the dangerous side effects and low efficacy. Therefore, using of herbal medicines is an important component of alternative and complementary medicine, Mesenchymal stem cells have a special site in the treatment of diseases. drugs.Psidium guajava Leaves used as antioxidant, anti-inflammatory antinociceptive and analgesic. The goals of scientific research of this study were to the evaluate the regeneration articular cartilage effect of injection of the hydroalcoholic extract of leave of   Psidium Guajava with Stem Cell  into the knee joints of rats.
Methods: Firstly, ninety male rats were choosen, and collagenase type II was prepared for an intra-articular knee injection, for them to induce OA in the animal models. Then, they were divided into nine treatment groups. (N = 10): (a) Sham, (b) Control, (c) injection of hyaluronic Acid  intra-articular, (d) Injections of Stem Cell  intra-articular, (e) Injections of PRP, (f): Injections of the extract Psidium guajava leaves  (g) Injections of the extract Psidium guajava leave with   PRP,    (h) Injections of the extract Psidium guajava leaves with Stem Cell  (i) Injections of the extract Psidium guajava leaves with Stem Cell and PRP. After 5 months, the results were assessments by a Radiologist and Histopathologist.
 Results: Radiological and histopathological findings were in which the improvement of the articular cartilage was better in the leaves with Stem Cell and PRP compared with the  control group(P<0.05).
Conclusions: It seems that Psidium guajava leaves can be suggested as an effective complementary and alternative treatment for OA of the knee. Because it has a lot of polyphenol compounds.

Keywords

osteoarthritis
knee
Psidium guajava
PRP
Stem Cell
Rat

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