Evaluation the effect of Pistacia atlantica hydro alcoholic extract with bone marrow- derived stem cells on induced knee osteoarthritis in rat

Borzooeian, Giti; Tanideh, Nader; Lotfi, Mehrzad; Mokhtari, Maral; Nazem, Habibollah; Sani, Mahsa; Salman-nezhad, Mahin; Koohi-hosseinabadi, Omid; Daneshi, Sajad

doi:10.22034/ijos.2020.121317

Evaluation the effect of Pistacia atlantica hydro alcoholic extract with bone marrow- derived stem cells on induced knee osteoarthritis in rat

Authors

giti Borzooeian

Nader Tanideh

Mehrzad Lotfi

Maral Mokhtari

Habibollah Nazem

Mahsa Sani

Mahin Salman-nezhad

omid koohi-hosseinabadi

sajad daneshi

https://doi.org/10.22034/ijos.2020.121317

Abstract

Background: Osteoarthritis is a progressive joint disease. Mastic fruit has antioxidant and anti-inflammatory properties. Bone marrow stem cells have the ability to differentiate into chondrocyte. The purpose of this study is evaluation the effect of Pistacia atlantica hydro alcoholic extract with bone marrow- derived stem cells on induced knee osteoarthritis in rat.

Methods: Firstly, 50 male Wistar rats were divided into 5 groups of 10. Then, on days zero and 3, fifty μl collagenase type 2 under anesthesia was injected in the left knee. After 10 weeks, osteoarthritis was induced and rats based on the type of treatment were divided into 5 groups including: control, treatment by stem cell and Pistacia atlantica hydro alcoholic extract, Pistacia atlantica hydro alcoholic extract therapy, stem cell therapy and treatment with hyalgan. At the end of study, radiographic and histopathology surveys and also measured of antioxidant properties as MDA-TAC was done.

Results: After 12 weeks of treatment, radiographic and histopathological evaluation showed that the results of the cell with extract group was significantly different compared to other treatment groups and the control group (P <0.05). Also, based on biochemical evaluation of MDA-TAC in cell with extract group significant reduction in oxidative stress is observed compared to other treatment groups and control (P <0.05).

Conclusions: It seems that Pistacia atlantica hydro alcoholic extract with bone marrow- derived stem cells are effective in knee cartilage repair.

Keywords

osteoarthritis

Pistacia atlantica Hydro Alcoholic Extract

Bone Marrow Stem Cells

Chondrocyte